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Oxidant stress and signal transduction in the nervous system with the PI 3-K, Akt, and mTOR cascade

International Journal of Biochemistry and Cell Biology, 28(10), 1085-1087

Serial 1:10, 1:100, 1:10,000, and 1:100,000 dilutions of each cell lysate sample in PBS and endotoxin-free water were generated and used for each of the assays below to ensure that protein and LPS levels were in the range of the standard curves